Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium

Acesulfame potassium (ACE-K) is an artificial sweetener widely used in many foods. This investigation assessed the cytotoxic effect of ACE-K using MTT assay human hepatocellular carcinoma (HepG2) cell line and genotoxic chromosomal aberrations (CAs), micronucleus (MN), comet assays lymphocytes. 7.5-240 ?g/mL concentrations were applied to cells. notably decreased viability on HepG2 cells, especially at 120 240 24 48 h. It also significantly reduced mitotic index (MI) 60, 120, both treatments (24 h) The frequency CAs increased for h treatment compared control. However, no difference was observed MN nuclear division (NDI) all treatments. induced tail length, intensity, moment 15 isolated Therefore, showed a cells as well lymphocytes higher concentrations. exhibits mild by increasing long-term DNA damaging only ?g/mL.